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Biosensors combine a biological recognition layer with a physicochemical transduction layer and an electronic signal processing device that can be employed as an analytical tool to detect an analyte in a wide range of environments [54]. As hybrid devices, they play an essential role by achieving rapid and selective quantitative or semiquantitative analysis when compared to conventional detection methods, such as chromatography and spectroscopy, which are usually costly and time consuming [47,55]. Furthermore, biosensors are low-cost devices that do not require extensive instrumentation and have relatively fast response times due to their chemical redox response [35,51,52,56].
Among the different transducers available to convert biochemical signals into measurable signals, electrochemical systems have frequently been used as the detection mode in commercial biosensors. Their quantification involves detecting a redox reaction in the transducer when the bioreceptor interacts selectively with the analyte in the solution, which generates an electrochemical signal. This signal can induce amperometric and potentiometric responses, field-effect transistors (FET), and conductometric responses [52,56]. The advances in biosensing analyses require understanding the charge transport and the electron transfer that occurs in the electrode/electrolyte interface, which will lead to an efficient bond between the bioreceptor and the target analyte, and will immobilize the biomolecule in the transducer [43,47,158].
Furthermore, the increased interest in wearable electronic devices has required flexible biosensors [203]. The substrate for fabricating this wearable sensing platform requires improved mechanical flexibility, chemical and thermal stability, biocompatibility, and conformal contact with the skin [191,204,205,206]. Paper-based biosensors, such as dipstick, lateral flow assay, and microfluidic paper-based analytical devices, have received significant attention as they allow for low-cost, portable, and disposable platforms. Nanopaper that is made entirely of BNC has all the advantageous features exhibited by conventional paper, such as versatility, abundance, transparency, flexibility, and cost. Nanopaper also obviates the drawbacks mentioned earlier by offering much lower thermal expansion and much higher chemical, mechanical, and thermal stability [40].
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Cell-free DNA (cfDNA) is easily accessible in peripheral blood and can be used as biomarkers for cancer diagnostics, prognostics, and therapeutics. With the advent of various technologies, including machine learning/AI-based methods of analysis, cfDNA market is presenting more and more opportunities in clinical oncology, particularly in cancer screening, early diagnosis, therapeutic evaluation, monitoring disease progression, and determining disease prognosis.
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